CYTOCHROME OXIDASE-CATALYZED SUPEROXIDE GENERATION FROM HYDROGEN-PEROXIDE

被引：42

作者：

KSENZENKO, MY

VYGODINA, TV

BERKA, V

RUUGE, EK

KONSTANTINOV, AA

机构：

[1] AN BELOZERSKY PHYSICOCHEM BIOL INST, MOSCOW 119899, USSR

[2] MV LOMONOSOV STATE UNIV, FAC PHYS, MOSCOW 119899, USSR

[3] SLOVAK ACAD SCI, INST EXPTL PHYS, KOSICE, CZECHOSLOVAKIA

来源：

FEBS LETTERS | 1992年 / 297卷 / 1-2期

关键词：

CYTOCHROME-C OXIDASE; SUPEROXIDE RADICAL; SPIN TRAPPING; OXYGEN INTERMEDIATE; RESPIRATORY CHAIN;

D O I：

10.1016/0014-5793(92)80328-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Superoxide dismutase is shown to affect spectral changes observed upon cytochrome c oxidase reaction with H2O2, which indicates a possibility of O2- radicals being formed in the reaction. Using DMPO as a spin trap, generation of superoxide radicals from H2O2 in the presence of cytochrome oxidase is directly demonstrated. The process is inhibited by cyanide and is not observed with a heat-denatured enzyme pointing to a specific reaction in the oxygen-reducing centre of cytochrome c oxidase. The data support a hypothesis on a catalase cycle catalyzed by cytochrome c oxidase in the presence of excess H2O2 (Vygodina and Konstantinov (1988) Ann. NY Acad. Sci., 550, 124-138): [GRAPHICS]

引用

页码：63 / 66

页数：4

共 24 条

[1] CYTOCHROME-C OXIDASE BINDING OF HYDROGEN-PEROXIDE [J].