RESOLUTION OF DIASTEREOMERIC PHOSPHORAMIDATE BRIDGED UNNATURAL OLIGONUCLEOTIDES BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Micellar electrokinetic chromatography was used to resolve diastereomers of oligonucleotides possessing several chiral phosphoramidate bridges. These materials were not resolved by conventional liquid chromatographic techniques. Parameters that were found to affect electrophoretic resolution included the nature and concentration of surfactant, presence of organic modifiers such as propan-2-ol and urea, use of deuterium oxide-based buffers and temperature control. Practical optimization was achieved by employing Terabe's resolution principles.