Helix packing in polytopic membrane proteins: Role of glycine in transmembrane helix association

被引:235
作者
Javadpour, MM
Eilers, M
Groesbeek, M
Smith, SO
机构
[1] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
[2] Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA
关键词
D O I
10.1016/S0006-3495(99)77009-8
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The nature and distribution of amino acids in the helix interfaces of four polytopic membrane proteins (cytochrome c oxidase, bacteriorhodopsin, the photosynthetic reaction center of Rhodobacter sphaeroides, and the potassium channel of Streptomyces lividans) are studied to address the role of glycine in transmembrane helix packing. In contrast to soluble proteins where glycine is a noted helix breaker, the backbone dihedral angles of glycine in transmembrane helices largely fall in the standard a-helical region of a Ramachandran plot. An analysis of helix packing reveals that glycine residues in the transmembrane region of these proteins are predominantly oriented toward helix-helix interfaces and have a high occurrence at helix crossing points. Moreover, packing Voids are generally not formed at the position of glycine in folded protein structures. This suggests that transmembrane glycine residues mediate helix-helix interactions in polytopic membrane proteins in a fashion similar to that seen in oligomers of membrane proteins with single membrane-spanning helices. The picture that emerges is one where glycine residues serve as molecular notches for orienting multiple helices in a folded protein complex.
引用
收藏
页码:1609 / 1618
页数:10
相关论文
共 38 条
[1]  
ANDERSEN JP, 1992, J BIOL CHEM, V267, P2767
[2]   RULES FOR ALPHA-HELIX TERMINATION BY GLYCINE [J].
AURORA, R ;
SRINIVASAN, R ;
ROSE, GD .
SCIENCE, 1994, 264 (5162) :1126-1130
[3]  
BORMANN BJ, 1989, J BIOL CHEM, V264, P4033
[4]   Helix packing in membrane proteins [J].
Bowie, JU .
JOURNAL OF MOLECULAR BIOLOGY, 1997, 272 (05) :780-789
[5]   Helix packing angle preferences [J].
Bowie, JU .
NATURE STRUCTURAL BIOLOGY, 1997, 4 (11) :915-917
[6]   Dimerization of the p185(neu) transmembrane domain is necessary but not sufficient for transformation [J].
Burke, CL ;
Lemmon, MA ;
Coren, BA ;
Engelman, DM ;
Stern, DF .
ONCOGENE, 1997, 14 (06) :687-696
[7]   STRUCTURAL INVARIANTS IN PROTEIN FOLDING [J].
CHOTHIA, C .
NATURE, 1975, 254 (5498) :304-308
[8]   HELIX TO HELIX PACKING IN PROTEINS [J].
CHOTHIA, C ;
LEVITT, M ;
RICHARDSON, D .
JOURNAL OF MOLECULAR BIOLOGY, 1981, 145 (01) :215-250
[9]   STRUCTURE OF PROTEINS - PACKING OF ALPHA-HELICES AND PLEATED SHEETS [J].
CHOTHIA, C ;
LEVITT, M ;
RICHARDSON, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (10) :4130-4134
[10]   ROLE OF TRANSMEMBRANE DOMAIN INTERACTIONS IN THE ASSEMBLY OF CLASS-II MHC MOLECULES [J].
COSSON, P ;
BONIFACINO, JS .
SCIENCE, 1992, 258 (5082) :659-662