MECHANISM OF PHYTOHEMAGGLUTININ(PHA) ACTION .I. MOUSE SPLEEN CELLS IN SHORT TERM CULTURE . EFFECT OF NEONATAL EXPOSURE TO PHYTOHEMAGGLUTININ

被引:18
作者
LINDAHLK.K
PETERSON, RD
机构
[1] Institutes for Medical Genetics, Zoophysiology and Histology, University of Uppsala, Uppsala
关键词
D O I
10.1016/0014-4827(69)90238-9
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The optimal conditions for stimulation of mouse spleen cells with phytohemagglutinin (PHA) in short term culture have been explored. A method for determination of the thymidine incorporation initiated by PHA is described. It is applied to a study of the PHA response in spleen cells from mice injected with PHA at birth compared to untreated mice of corresponding age. © 1969.
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页码:231 / &
相关论文
共 20 条
[1]   PURIFICATION OF MITOSIS-STIMULATING FACTOR FROM PHASEOLUS VULGARIS [J].
BORJESON, J ;
BOUVENG, R ;
NORDEN, A ;
GARDELL, S ;
THUNELL, S .
BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 82 (01) :158-&
[3]   REACTION OF HUMAN LYMPHOCYTES IN CULTURE TO COMPONENTS OF MEDIUM [J].
JOHNSON, GJ ;
RUSSELL, PS .
NATURE, 1965, 208 (5008) :343-&
[4]   RAPID ACTIVATION OF LYMPHOCYTES BY PHYTOHAEMAGGLUTININ [J].
KAY, JE .
NATURE, 1967, 215 (5102) :737-&
[5]  
KNIGHT S, 1965, IMMUNOLOGY, V9, P565
[6]   THE MECHANISM OF CELL BINDING AND CELL-SHEET FORMATION IN L-STRAIN FIBROBLASTS [J].
KUCHLER, RJ ;
MARLOWE, ML ;
MERCHANT, DJ .
EXPERIMENTAL CELL RESEARCH, 1960, 20 (02) :428-437
[7]  
LINDAHLKIESSLIN.K, 1964, LANCET, V2, P591
[8]  
LINDAHLKIESSLIN.K, 1963, 11 P INT C GEN, P300
[9]  
MARSHALL WH, 1963, LANCET, V1, P773