DIFFERENTIAL REGULATION OF BLADDER BETA-ADRENERGIC AND MUSCARINIC CHOLINERGIC RECEPTORS IN EXPERIMENTAL DIABETES

被引:29
作者
LATIFPOUR, J
NISHIMOTO, T
MARIAN, MJ
YOSHIDA, M
WEISS, RM
机构
关键词
D O I
10.2337/diabetes.40.9.1150
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To determine the contribution of diuresis-induced bladder hypertrophy, which accompanies the diabetic state, on the biochemical and functional alterations observed in the diabetic bladder, we compared three experimental groups: 8-wk streptozocin (STZ)-induced diabetic rats, 8-wk sucrose-fed diuretic rats, and age-matched controls. Diabetic and sucrose-fed rats had higher water intake, higher urine output, and larger bladders than controls. Diabetic rats had lower serum insulin levels, lower body weights, and higher serum glucose levels than either control or sucrose-fed animals. Receptor binding studies with [H-3]quinuclidinyl benzilate in bladder dome demonstrated an upregulation of muscarinic receptors in diabetic and sucrose-fed rats compared with controls. Parallel binding studies with [H-3]dihydroalprenolol and [I-125]iodopindolol showed an upregulation of beta-adrenergic receptors in diabetic but not in sucrose-fed bladder domes. Carbachol induced larger contractile responses in diabetic and sucrose-fed than in control bladder dome muscle strips. Isoproterenol relaxed KCl-contracted detrusor strips from both diabetic and sucrose-fed rats to a greater degree and with a higher affinity than detrusor strips from controls. Our data show that overdistension and increased workload per se contributed to the upregulation of muscarinic but not to the upregulation of beta-adrenergic receptors in STZ-induced diabetes. Furthermore, the magnitude of carbachol-induced contractions correlated with muscarinic receptor upregulation, whereas the magnitude of isoproterenol-induced relaxation did not correlate with changes in the density of the beta-adrenergic receptors. Thus, it appears that different regulatory mechanisms are involved in diabetes-induced alterations in muscarinic and beta-adrenergic receptors in bladder dome.
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页码:1150 / 1156
页数:7
相关论文
共 42 条
[1]   CYSTOMETRICAL AND INVITRO EVALUATION OF URINARY-BLADDER FUNCTION IN RATS WITH STREPTOZOTOCIN-INDUCED DIABETES [J].
ANDERSSON, PO ;
MALMGREN, A ;
UVELIUS, B .
JOURNAL OF UROLOGY, 1988, 139 (06) :1359-1362
[2]   INSULIN PREVENTION OF ALTERED MUSCARINIC RECEPTOR-G PROTEIN COUPLING IN DIABETIC RAT ATRIA [J].
ARONSTAM, RS ;
CARRIER, GO .
DIABETES, 1989, 38 (12) :1611-1616
[3]   DIABETIC BLADDER [J].
BUCK, AC ;
MCRAE, CU ;
REED, PI ;
CHISHOLM, GD .
PROCEEDINGS OF THE ROYAL SOCIETY OF MEDICINE-LONDON, 1974, 67 (01) :81-83
[4]   BLADDER DYSFUNCTION AND NEUROPATHY IN DIABETES [J].
BUCK, AC ;
REED, PI ;
SIDDIQ, YK ;
CHISHOLM, GD ;
FRASER, TR .
DIABETOLOGIA, 1976, 12 (03) :251-258
[5]   IMPAIRMENT AND RESTORATION OF RAT URINARY-BLADDER RESPONSIVENESS FOLLOWING DISTENSION [J].
CARPENTER, FG .
AMERICAN JOURNAL OF PHYSIOLOGY, 1983, 244 (01) :R106-R113
[6]   THEORETICAL BASES FOR VASCULAR SELECTIVITY OF CA-2+ ANTAGONISTS [J].
CAUVIN, C ;
LUKEMAN, S ;
CAMERON, J ;
HWANG, O ;
MEISHERI, K ;
YAMAMOTO, H ;
VANBREEMEN, C .
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY, 1984, 6 :S630-S638
[7]   BETA-ADRENERGIC RECEPTORS AND ADENYLATE-CYCLASE ACTIVITY IN DIABETIC RAT FAT-CELLS [J].
DECINGOLANI, GEC .
DIABETES, 1986, 35 (11) :1229-1232
[8]  
DELEAN A, 1982, MOL PHARMACOL, V21, P5
[9]  
EKSTROM J, 1984, ACTA PHYSIOL SCAND, V122, P45, DOI 10.1111/j.1748-1716.1984.tb07479.x
[10]   LENGTH-TENSION RELATIONS OF SMOOTH-MUSCLE FROM NORMAL AND DENERVATED RAT URINARY BLADDERS [J].
EKSTROM, J ;
UVELIUS, B .
ACTA PHYSIOLOGICA SCANDINAVICA, 1981, 112 (04) :443-447