CONTROL OF YEAST GAL GENES BY MIG1 REPRESSOR - A TRANSCRIPTIONAL CASCADE IN THE GLUCOSE RESPONSE

被引：329

作者：

NEHLIN, JO

CARLBERG, M

RONNE, H

机构：

[1] Ludwig Inst. for Cancer Resch., Uppsala Branch, Biomedical Center, S-751 24 Uppsala

来源：

EMBO JOURNAL | 1991年 / 10卷 / 11期

关键词：

GENE EXPRESSION; GLUCOSE REPRESSION; SACCHAROMYCES-CEREVISIAE; TRANSCRIPTION; ZINC FINGER PROTEIN;

D O I：

10.1002/j.1460-2075.1991.tb04901.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose repression is a global regulatory mechanism in yeast. We have investigated how glucose regulates the GAL genes, which are required for galactose fermentation. We found that the GAL genes are controlled by a transcriptional cascade. Thus, GAL4, which encodes an activator of the GAL genes, is repressed by MIG1, a zinc finger protein that binds to the GAL4 promoter. MIG1 has a dual role in control of the GAL genes, since MIG1 also binds to the promoter of GAL1, a gene regulated by GAL4. A disruption of MIG1 interacts synergistically with a disruption of GAL80, a gene involved in galactose induction. This suggests that the MIG1-dependent response to glucose is amplified by down-regulation of the induction pathway.

引用

页码：3373 / 3377

页数：5

共 33 条

[1] YEAST REGULATORY GENE GAL3 - CARBON REGULATION - UASGAL ELEMENTS IN COMMON WITH GAL1, GAL2, GAL7, GAL10, GAL80, AND MEL1 - ENCODED PROTEIN STRIKINGLY SIMILAR TO YEAST AND ESCHERICHIA-COLI GALACTOKINASES [J].