EXPLOITING STOPPED-FLOW INJECTION METHODS FOR QUANTITATIVE CHEMICAL-ASSAYS

Advantages of the stopped-flow injection techniques are emphasized and illustrated by examples of assays developed in the authors' laboratory, including enzymatic measurements for bioprocess monitoring, the monitoring of enzyme reactions on solid surfaces, the determination of reaction rate constants, membrane preconcentration of molecular species, flow-injection-Fourier transform IR measurements and a novel type of flow-injection coulometric titration. Sequential injection analysis, a next generation of stopped-flow injection methodologies, is discussed together with new ways of propelling and controlling flows, including novel syringe-based devices.