DELETION OF THE ESSENTIAL GENE 24 FROM THE BACTERIOPHAGE-T4 GENOME

被引:6
作者
ENGMAN, HW
KREUZER, KN
机构
[1] DUKE UNIV,MED CTR,DEPT MICROBIOL,BOX 3020,DURHAM,NC 27710
[2] DUKE UNIV,PROGRAM GENET,DURHAM,NC 27710
关键词
GENETIC MARKER; DELETION MUTANT; LATE TRANSCRIPTION; RECOMBINATION; REPLICATION ORIGIN; HEAD PROTEIN;
D O I
10.1016/0378-1119(93)90541-A
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
We deleted the essential gene 24 from the genome of bacteriophage T4. The DELTA24 phage is a conditional lethal mutant that can grow only when the host strain supplies the product of gene 24 in trans, or when the phage acquires a functional gene 24 by some type of recombination event. Thus, gene 24 can be used as a selectable marker, for example permitting transposition into the T4 genome and analyses of plasmid-phage recombination [Woodworth and Kreuzer, Mol. Microbiol. 6 (1992) 1289-1296,- H.W.E. and K.N.K., manuscript submitted]. We also found that the promoter region of gene 24 allows a low level of autonomous plasmid replication in T4-infected cells, raising the possibility of a previously unrecognized mode of T4 replication initiation.
引用
收藏
页码:69 / 74
页数:6
相关论文
共 21 条
[1]   THE COMPLEX PATTERN OF TRANSCRIPTION IN THE SEGMENT OF THE BACTERIOPHAGE-T4 GENOME CONTAINING 3 OF THE HEAD PROTEIN GENES [J].
ELLIOTT, T ;
KASSAVETIS, GA ;
GEIDUSCHEK, EP .
VIROLOGY, 1984, 139 (02) :260-282
[2]  
GEIDUSCHEK EP, 1991, ANNU REV GENET, V25, P437
[3]  
GEIDUSCHEK EP, 1983, BACTERIOPHAGE T4, P189
[4]   ENHANCEMENT OF BACTERIOPHAGE-T4 LATE TRANSCRIPTION BY COMPONENTS OF THE T4 DNA-REPLICATION APPARATUS [J].
HERENDEEN, DR ;
KASSAVETIS, GA ;
BARRY, J ;
ALBERTS, BM ;
GEIDUSCHEK, EP .
SCIENCE, 1989, 245 (4921) :952-958
[5]   AN RNA POLYMERASE-BINDING PROTEIN THAT IS REQUIRED FOR COMMUNICATION BETWEEN AN ENHANCER AND A PROMOTER [J].
HERENDEEN, DR ;
WILLIAMS, KP ;
KASSAVETIS, GA ;
GEIDUSCHEK, EP .
SCIENCE, 1990, 248 (4955) :573-578
[6]   GENETIC COMPLEMENTATION BY CLONED BACTERIOPHAGE-T4 LATE GENES [J].
JACOBS, KA ;
ALBRIGHT, LM ;
SHIBATA, DK ;
GEIDUSCHEK, EP .
JOURNAL OF VIROLOGY, 1981, 39 (01) :31-45
[7]   REGULATION OF EXPRESSION OF CLONED BACTERIOPHAGE-T4 LATE GENE-23 [J].
JACOBS, KA ;
GEIDUSCHEK, EP .
JOURNAL OF VIROLOGY, 1981, 39 (01) :46-59
[8]   REGULATION OF TRANSCRIPTION IN THE SEGMENT OF THE BACTERIOPHAGE-T4 GENOME CONTAINING 3 OF THE HEAD PROTEIN GENES - PLASMID-SPECIFIC AND PHAGE CHROMOSOME-SPECIFIC EFFECTS [J].
KASSAVETIS, GA ;
ELLIOTT, T ;
GEIDUSCHEK, EP .
VIROLOGY, 1984, 139 (02) :283-294
[9]   A DEFECTIVE PHAGE SYSTEM REVEALS BACTERIOPHAGE-T4 REPLICATION ORIGINS THAT COINCIDE WITH RECOMBINATION HOT SPOTS [J].
KREUZER, KN ;
ALBERTS, BM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (10) :3345-3349
[10]  
KREUZER KN, 1988, J BIOL CHEM, V263, P11366