SELECTION OF A CHEMICALLY DEFINED MEDIUM FOR CULTURING ADULT NEWT FORELIMB REGENERATES

Three commercially available tissue-culture media were evaluated for their ability to support continued growth and differentiation of 14-day regenerates of adult newt forelimbs. Serums, embryo extracts, egg ultrafiltrates, and antimicrobial agents were avoided in this analysis. The hormones insulin and l-thyroxine were added to these chemically defined media to enhance continued cellular metabolism and growth. The optimum conditions appeared to be cultivation at 25° C (pH 7.2 to 7.4) in media osmotically adjusted to conditions approximating amphibian blood values (i.e. 225 m0 sm for 199, 244 m0 sm for CMRL-1066, and 262 m0 sm for L-15). © 1979 Tissue Culture Association.