RELATIONSHIP BETWEEN MIGRATION-INHIBITION AND PLAQUE-FORMING CELL RESPONSES TO SHEEP ERYTHROCYTES IN THE TELEOST, TILAPIA-MOSSAMBICA

Cell-mediated immunity to sheep erythrocytes (SRBC) in the teleost, Tilapia mossambica was assessed using the in vitro capillary migration inhibition (MI) assay and the humoral immune response by the plaque-forming cell (PFC) assay. Both PFC and MI responses were found to be inversely proportional to the amount of antigen injected. Cyclophosphamide administration immediately after immunization completely abrogated both PFC and MI responses. In contrast, injection of formalinized SRBC resulted in greater elevation of MI levels accompanied by the absence of PFC production. The possible regulatory role of antibody-producing cells in the MI response to SRBC is discussed. © 1979 Pergamon Press Ltd. All rights reserved.