EFFECTS OF SURFACTANT SUBFRACTIONS ON PRETERM RABBIT LUNG-FUNCTION

被引:69
作者
YAMADA, T [1 ]
IKEGAMI, M [1 ]
JOBE, AH [1 ]
机构
[1] UNIV CALIF LOS ANGELES,LOS ANGELES CTY HARBOR MED CTR,DEPT PEDIAT,DIV NEONATOL,A-17 ANNEX,TORRANCE,CA 90509
关键词
D O I
10.1203/00006450-199006000-00011
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Surfactant isolated from adult rabbits was separated into large aggregate forms (LA) and small aggregate forms (SA) by centrifugation at 40 000 × g. LA contain SP-A and the surfactant lipophilic proteins SP-B and SP-C and are surface-active in vitro, whereas SA lack the surfactant proteins and have poor surface properties. Premature rabbits were treated with LA or SA and ventilated to evaluate the effects of LA and SA in vivo. There were dose-response effects of LA at doses between 10 and 50 mg total lipid/kg: dynamic compliances increased from 0.4 to 0.8 mL/cm H20/kg and pressure-volume curves improved significantly, whereas SA did not improve any of the measurements at any dose. Organic solvent extracts of SA made up into LA also did not improve in vivo function. When 1.5% SP-B and SP-C isolated from calf surfactant was added to organic solvent extracts of SA, the surfactant had good in vitro surface properties and increased maximum lung volumes. However, this surfactant did not improve dynamic compliances or lung stability on pressure-volume curves. Because the lipids from SA seemed to limit the compliance response, we tried to identify differences in lipids from LA and SA. Phospholipids from SA reconstituted with SP-BC did not function well in vivo, whereas phospholipids from LA with SP-BC were similar to LA surfactant. The neutral lipid fractions from LA or SA did not contribute very much to or inhibit function. Fatty acid composition of the phospholipids and phosphatidylcholine from LA and SA were not remarkably different. We conclude that subtle compositional differences in the phospholipids or difficulties with reconstriction of the lipids with SP-BC prevents in vivo function of SA surfactant. © 1990 International Pediatric Research Foundation, Inc. All Rights Reserved.
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页码:592 / 598
页数:7
相关论文
共 39 条
[1]   HETEROGENEITY OF ALVEOLAR SURFACTANT IN THE RABBIT - COMPOSITION, MORPHOLOGY, AND LABELING OF SUBFRACTIONS ISOLATED BY CENTRIFUGATION OF LUNG LAVAGE [J].
BARITUSSIO, A ;
BELLINA, L ;
CARRARO, R ;
ROSSI, A ;
ENZI, G ;
MAGOON, MW ;
MUSSINI, I .
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1984, 14 (01) :24-29
[2]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[3]   POSTNATAL TRANSFORMATIONS OF ALVEOLAR SURFACTANT IN THE RABBIT - CHANGES IN POOL SIZE, POOL MORPHOLOGY AND ISOFORMS OF THE 32-38 KDA APOLIPOPROTEIN [J].
BRUNI, R ;
BARITUSSIO, A ;
QUAGLINO, D ;
GABELLI, C ;
BENEVENTO, M ;
RONCHETTI, IP .
BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 958 (02) :255-267
[4]   SURFACTANT SUBTYPES OF MICE - METABOLIC RELATIONSHIPS AND CONVERSION INVITRO [J].
GROSS, NJ ;
NARINE, KR .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 67 (01) :414-421
[5]   SURFACTANT SUBTYPES IN MICE - CHARACTERIZATION AND QUANTITATION [J].
GROSS, NJ ;
NARINE, KR .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 66 (01) :342-349
[6]   NUCLEOTIDE AND AMINO-ACID-SEQUENCES OF PULMONARY SURFACTANT PROTEIN SP-18 AND EVIDENCE FOR COOPERATION BETWEEN SP-18 AND SP 28-36 IN SURFACTANT LIPID ADSORPTION [J].
HAWGOOD, S ;
BENSON, BJ ;
SCHILLING, J ;
DAMM, D ;
CLEMENTS, JA ;
WHITE, RT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (01) :66-70
[7]   EFFECTS OF A SURFACTANT-ASSOCIATED PROTEIN AND CALCIUM-IONS ON THE STRUCTURE AND SURFACE-ACTIVITY OF LUNG SURFACTANT LIPIDS [J].
HAWGOOD, S ;
BENSON, BJ ;
HAMILTON, RL .
BIOCHEMISTRY, 1985, 24 (01) :184-190
[8]  
HORBAR JD, 1989, NEW ENGL J MED, V320, P959
[9]   SURFACTANT METABOLISM IN SURFACTANT-TREATED PRETERM VENTILATED LAMBS [J].
IKEGAMI, M ;
JOBE, A ;
YAMADA, T ;
PRIESTLY, A ;
RUFFINI, L ;
RIDER, E ;
SEIDNER, S .
JOURNAL OF APPLIED PHYSIOLOGY, 1989, 67 (01) :429-437
[10]   CORTICOSTEROIDS AND SURFACTANT CHANGE LUNG-FUNCTION AND PROTEIN LEAKS IN THE LUNGS OF VENTILATED PREMATURE RABBITS [J].
IKEGAMI, M ;
BERRY, D ;
ELKADY, T ;
PETTENAZZO, A ;
SEIDNER, S ;
JOBE, A .
JOURNAL OF CLINICAL INVESTIGATION, 1987, 79 (05) :1371-1378