EVALUATION OF CULTURE MEDIA FOR THE ISOLATION OF SALMONELLAS FROM SEWAGE SLUDGE

被引:15
作者
EDGAR, D
SOAR, MS
机构
[1] Ministry of Agriculture Fisheries and Food, Veterinary Investigation Centre, Newcastle Upon Tyne, Whitley Road, Longbenton
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1979年 / 47卷 / 02期
关键词
D O I
10.1111/j.1365-2672.1979.tb01750.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Ten samples of sewage sludge were examined by various methods for the isolation of salmonellas using three types of enrichment broth: Muller‐Kauffmann Tetrathionate Broth (MKTB), Selenite F Broth (SFB), and Brilliant Green Broth (BGB), two temperatures (37°C and 43°C) and three selective media: Deoxycholate Citrate Agar incubated aerobically (DCA), and anaerobically (DCA(N)), Brilliant Green Agar (BGA), and Bismuth Sulphite Agar (BSA). The results suggest that a combination of pre‐enriched MKTB incubated at 37°C and plated on to BGA at 24 and 48 h was the best method, but when examining contaminated material such as sewage sludge, it appears unwise to rely on one single method. Copyright © 1979, Wiley Blackwell. All rights reserved
引用
收藏
页码:237 / 241
页数:5
相关论文
共 16 条
  • [1] ARGENT VA, 1977, J I WATER POLLUT CON, V4, P511
  • [2] COOK G. T., 1951, MONTH BULL MED RES COUNC [GR BRIT], V10, P89
  • [3] COMPARISON OF 2 MODIFICATIONS OF BISMUTHSULPHITE AGAR FOR THE ISOLATION AND GROWTH OF SALMONELLA-TYPHI AND SALM-TYPHI-MURIUM
    COOK, GT
    [J]. JOURNAL OF PATHOLOGY AND BACTERIOLOGY, 1952, 64 (03): : 559 - 566
  • [4] EDEL W, 1973, B WORLD HEALTH ORGAN, V48, P167
  • [5] EDEL W, 1969, B WORLD HEALTH ORGAN, V41, P297
  • [6] HARVEY R W, 1953, Mon Bull Minist Health Public Health Lab Serv, V12, P149
  • [7] SALMONELLA SURVEILLANCE WITH REFERENCE TO PIGS - CARDIFF ABATTOIR, 1968-1975
    HARVEY, RWS
    PRICE, TH
    MORGAN, J
    [J]. JOURNAL OF HYGIENE, 1977, 78 (03) : 439 - 448
  • [8] HOBBS B C, 1945, Mon Bull Minist Health Emerg Public Health Lab Serv, V4, P63
  • [9] New selenite enrichment media for the isolation of typhoid and paratyphoid (salmonella) bacilli
    Leifson, E
    [J]. AMERICAN JOURNAL OF HYGIENE, 1936, 24 (02): : 423 - 432
  • [10] ISOLATION OF SALMONELLAE
    MCCOY, JH
    [J]. JOURNAL OF APPLIED BACTERIOLOGY, 1962, 25 (02): : 213 - &