CHROMATOFOCUSING OF AMINOACYL-TRANSFER RNA-SYNTHETASES, EXTRACTED FROM NMRI MOUSE-LIVER

被引：12

作者：

BERG, BH

机构：

[1] Department of Biochemistry, University of Bergen, Bergen

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1038卷 / 03期

关键词：

Aminoacyl-tRNA synthetases; Chromatofocusing; Isoelectric point; Phosphorylation;

D O I：

10.1016/0167-4838(90)90254-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatofocusing of 17 aminoacyl-tRNA synthetases extracted from NMRI mouse liver is described and the apparent isoelectric points of these enzymes are presented. Each of 15 aminoacyl-tRNA synthetases was present in two peaks. Isoleucyl-tRNA synthetase showed only one peak and arginyl-tRNA synthetase was present in three peaks. Phosphorylation/ dephosphorylation experiments with arginyl-tRNA synthetase indicate that the peaks represent phosphorylated and unphosphorylated synthetase protein. One example of detection of increased protein phosphorylation during a biological experiment is presented. © 1990.

引用

页码：391 / 394

页数：4

共 10 条

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