ENZYMATIC MICRO-DETERMINATION OF SERUM FREE FATTY-ACIDS

被引：254

作者：

SHIMIZU, S

INOUE, K

TANI, Y

YAMADA, H

机构：

[1] Department of Agricultural Chemistry, Kyoto University, Kyoto

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90151-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reliable, simple, and rapid enzymatic method is described for the microdetermination of serum free fatty acids. The principle of the method is based on the activation of free fatty acids by a bacterial acyl-CoA synthetase (EC 6.2.1.3). The reaction is followed as production of AMP using the myokinase-pyruvate kinase-lactate dehydrogenase system as an indicator reaction. Results on the determination using human and rabbit sera showed a close correlation with the chemical colorimetric method. © 1979.

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页码：341 / 345

页数：5

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