QUANTITATIVE CYTOCHEMICAL STUDY OF ACID-PHOSPHATASES IN RAT-LIVER PARENCHYMAL-CELLS OF DIFFERENT PLOIDY VALUES

The Vickers M86 integrating microdensitometer has been used to quantify the cytochemical reaction for acid naphthol AS-BI phosphatase activity in isolated, rat liver parenchymal cells. Data are presented which valudate the method. The use of this method together with that of the Feulgen reaction to estimate nuclear ploidy value in the same cell, has shown that there is an increase in acid phosphatase activity of up to 100% when the euploidy value of the liver cell doubles. It has been further shown that 70-80% of this enzyme activity is ouabain-sensitive, regardless of the euploidy value. The data may be interpreted to indicate that the extra gene copies of the polyploid cells are operative. © 1979 Chapman and Hall Ltd.