SOLUBILIZATION AND PARTIAL-PURIFICATION OF SQUALENE SYNTHASE FROM DAFFODIL MICROSOMAL-MEMBRANES

被引：11

作者：

BELINGHERI, L

BEYER, P

KLEINIG, H

GLEIZES, M

机构：

[1] UNIV BORDEAUX 1, PHYSIOL CELLULAIRE VEGETALE LAB, AVE FAC, F-33405 TALENCE, FRANCE

[2] UNIV FREIBURG, INST BIOL 2, W-7800 FREIBURG, GERMANY

来源：

FEBS LETTERS | 1991年 / 292卷 / 1-2期

关键词：

NARCISSUS-PSEUDONARCISSUS; SQUALENE SYNTHASE; CHAPS; MICROSOME;

D O I：

10.1016/0014-5793(91)80827-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A squalene synthase was solubilized from daffodil (Narcissus pseudonarcissus L.) microsomes with CHAPS, a zwitterionic non-denaturating detergent. By successive chromatography on DEAE Sephacel and APP Sepharose a fraction enriched in this enzyme (21-fold) was prepared.

引用

页码：34 / 36

页数：3

共 14 条

[1]

AGNEW WS, 1978, J BIOL CHEM, V253, P4574

[2] ISOLATION BY AN AQUEOUS 2-POLYMER PHASE SYSTEM AND IDENTIFICATION OF ENDOMEMBRANES FROM CITROFORTUNELLA MITIS FRUITS FOR SESQUITERPENE HYDROCARBON SYNTHESIS [J].