INVOLVEMENT OF MICROBODIES IN PENICILLIN BIOSYNTHESIS

被引：99

作者：

MULLER, WH ^{[1
]}

BOVENBERG, RAL ^{[1
]}

GROOTHUIS, MH ^{[1
]}

KATTEVILDER, F ^{[1
]}

SMAAL, EB ^{[1
]}

VANDERVOORT, LHM ^{[1
]}

VERKLEIJ, AJ ^{[1
]}

机构：

[1] ROYAL GIST BROCADES NV,RES & DEV,DELFT,NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1116卷 / 02期

关键词：

MICROBODY; ACYLTRANSFERASE; PENICILLIN BIOSYNTHESIS; SITE-DIRECTED MUTAGENESIS; IMMONUGOLD LABELING; ELECTRON MICROSCOPY; (PENICILLIUM-CHRYSOGENUM);

D O I：

10.1016/0304-4165(92)90118-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Penicillium chrysogenum strains were constructed which express a mutant acyltransferase lacking the putative targeting signal for microbody proteins. The mutated enzyme was located in vacuoles and in neighbouring cytoplasm. Although acyltransferase was expressed in vivo and was active in vitro, the mutants did not produce penicillin. The results demonstrate the involvement of microbodies in penicillin production.

引用

收藏

页码：210 / 213

页数：4

相关论文

共 11 条

[1] PURIFICATION TO HOMOGENEITY AND CHARACTERIZATION OF ACYL COENZYME-A-6-AMINOPENICILLANIC ACID ACYLTRANSFERASE OF PENICILLIUM-CHRYSOGENUM [J].

ALVAREZ, E ;

CANTORAL, JM ;

BARREDO, JL ;

DIEZ, B ;

MARTIN, JF .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1987, 31 (11) :1675-1682

[2] CLONING AND CHARACTERIZATION OF THE ACYL-COENZYME-A-6-AMINOPENICILLANIC-ACID-ACYLTRANSFERASE GENE OF PENICILLIUM-CHRYSOGENUM [J].

BARREDO, JL ;

VANSOLINGEN, P ;

DIEZ, B ;

ALVAREZ, E ;

CANTORAL, JM ;

KATTEVILDER, A ;

SMAAL, EB ;

GROENEN, MAM ;

VEENSTRA, AE ;

MARTIN, JF .

GENE, 1989, 83 (02) :291-300

[3] CASSETTES OF THE STREPTOALLOTEICHUS-HINDUSTANUS BLE GENE FOR TRANSFORMATION OF LOWER AND HIGHER EUKARYOTES TO PHLEOMYCIN RESISTANCE [J].

DROCOURT, D ;

CALMELS, T ;

REYNES, JP ;

BARON, M ;

TIRABY, G .

NUCLEIC ACIDS RESEARCH, 1990, 18 (13) :4009-4009

[4] A CONSERVED TRIPEPTIDE SORTS PROTEINS TO PEROXISOMES [J].

GOULD, SJ ;

KELLER, GA ;

HOSKEN, N ;

WILKINSON, J ;

SUBRAMANI, S .

JOURNAL OF CELL BIOLOGY, 1989, 108 (05) :1657-1664

[5] DISTRIBUTION OF GLYOXYLATE-CYCLE ENZYMES BETWEEN MICROBODIES AND MITOCHONDRIA IN ASPERGILLUS-TAMARII [J].

GRAVES, LB ;

ARMENTROUT, VN ;

MAXWELL, DP .

PLANTA, 1976, 132 (02) :143-148

[6] DIRECT EVALUATION OF PHENYLACETYL-COA - 6-AMINOPENICILLANIC ACID ACYLTRANSFERASE OF PENICILLIUM-CHRYSOGENUM BY BIOASSAY [J].

LUENGO, JM ;

IRISO, JL ;

LOPEZNIETO, MJ .

JOURNAL OF ANTIBIOTICS, 1986, 39 (11) :1565-1573

[7]

MAXWELL DP, 1973, PLANTA, V124, P109

[8] LOCALIZATION OF THE PATHWAY OF THE PENICILLIN BIOSYNTHESIS IN PENICILLIUM-CHRYSOGENUM [J].

MULLER, WH ;

VANDERKRIFT, TP ;

KROUWER, AJJ ;

WOSTEN, HAB ;

VANDERVOORT, LHM ;

SMAAL, EB ;

VERKLEIJ, AJ .

EMBO JOURNAL, 1991, 10 (02) :489-495

[9]

MULLER WH, 1991, J MICROSC-OXFORD, V164, P29

[10] EFFICIENT OLIGONUCLEOTIDE-DIRECTED CONSTRUCTION OF MUTATIONS IN EXPRESSION VECTORS BY THE GAPPED DUPLEX DNA METHOD USING ALTERNATING SELECTABLE MARKERS [J].

STANSSENS, P ;

OPSOMER, C ;

MCKEOWN, YM ;

KRAMER, W ;

ZABEAU, M ;

FRITZ, HJ .

NUCLEIC ACIDS RESEARCH, 1989, 17 (12) :4441-4454