EXPRESSION OF TGF-BETA-S AND TGF-BETA TYPE-II RECEPTOR MESSENGER-RNAS IN MOUSE FOLLICULOGENESIS - STORED MATERNAL TGF-BETA-2 MESSAGE IN OOCYTES

被引：37

作者：

SCHMID, P ^{[1
]}

COX, D ^{[1
]}

VANDERPUTTEN, H ^{[1
]}

MCMASTER, GK ^{[1
]}

BILBE, G ^{[1
]}

机构：

[1] CIBA GEIGY AG,DIV PHARMA,CH-4002 BASEL,SWITZERLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 02期

关键词：

D O I：

10.1006/bbrc.1994.1750

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present in situ hybridisation study describes expression of TGF-nl, TGF-beta 2, TGF-beta 3, and TGF-beta type II receptor mRNA during follicular development, and the temporal pattern and abundance of TGF-beta 2 transcripts in early pre-implantation embryos. TGF-beta 1 hybridisation signals were most prominent in the outer granulosa cell layers of the post-antral follicles and in the corpus luteum, whereas strong expression of TGF-beta type II receptor mRNA was confined to thecal cells. Weak TGF-beta 3 mRNA expression was observed in all major cell types of pre- and post-antral follicles. Most notably, the very strong TGF-beta 2 hybridisation signals which were detected in developing oocytes declined rapidly following fertilization. Although still abundant in two-cell embryos, TGF-beta 2 hybridisation signals were barely detectable in four- and eight-cell embryos. These findings, and the presence of specific sequence motifs in the 3' untranslated region of TGF-beta 2 mRNAs, suggest that TGF-beta 2 trancripts are stored as maternal messages. (C) 1994 Academic Press, Inc.

引用

页码：649 / 656

页数：8

共 21 条

[1] OVARIAN TRANSFORMING GROWTH FACTOR-BETA (TGF-BETA) - CELLULAR SITE(S), AND MECHANISM(S) OF ACTION [J].