THE PROTEIN IMPORT RECEPTOR MOM19 OF YEAST MITOCHONDRIA

被引：54

作者：

MOCZKO, M ^{[1
]}

GARTNER, F ^{[1
]}

PFANNER, N ^{[1
]}

机构：

[1] UNIV FREIBURG, INST BIOCHEM, HERMANN HERDER STR 7, D-79104 FREIBURG, GERMANY

来源：

FEBS LETTERS | 1993年 / 326卷 / 1-3期

关键词：

MITOCHONDRION; RECEPTOR; MOM19; PREPROTEIN; PROTEIN TRANSLOCATION;

D O I：

10.1016/0014-5793(93)81801-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified the protein import receptor MOM19 of Saccharomyces cerevisiae mitochondria. MOM19 is exposed on the outer membrane surface and present in the mitochondrial receptor complex. Antibodies raised against MOM19 strongly inhibited the import of preproteins into isolated yeast mitochondria. Fab fragments prepared from the antibodies showed the same inhibitory effect. By using mutant mitochondria, which lacked the second import receptor MOM72, we found that the import of preproteins via MOM19 did not require the presence of MOM72. We conclude that MOM19 is required for preprotein translocation across the yeast mitochondrial outer membrane and is able to function independently of the receptor MOM72.

引用

页码：251 / 254

页数：4

共 18 条

[1] A YEAST MITOCHONDRIAL OUTER-MEMBRANE PROTEIN ESSENTIAL FOR PROTEIN IMPORT AND CELL VIABILITY [J].

BAKER, KP ;

SCHANIEL, A ;

VESTWEBER, D ;

SCHATZ, G .

NATURE, 1990, 348 (6302) :605-609

[2]

HINES V, 1993, J BIOL CHEM, V268, P449

[3] PROTEIN IMPORT INTO YEAST MITOCHONDRIA IS ACCELERATED BY THE OUTER-MEMBRANE PROTEIN MAS70 [J].

HINES, V ;

BRANDT, A ;

GRIFFITHS, G ;

HORSTMANN, H ;

BRUTSCH, H ;

SCHATZ, G .

EMBO JOURNAL, 1990, 9 (10) :3191-3200

[4] INSERTION OF MOM22 INTO THE MITOCHONDRIAL OUTER-MEMBRANE STRICTLY DEPENDS ON SURFACE-RECEPTORS [J].

KEIL, P ;

PFANNER, N .

FEBS LETTERS, 1993, 321 (2-3) :197-200

[5] IDENTIFICATION OF A MITOCHONDRIAL RECEPTOR COMPLEX REQUIRED FOR RECOGNITION AND MEMBRANE INSERTION OF PRECURSOR PROTEINS [J].

KIEBLER, M ;

PFALLER, R ;

SOLLNER, T ;

GRIFFITHS, G ;

HORSTMANN, H ;

PFANNER, N ;

NEUPERT, W .

NATURE, 1990, 348 (6302) :610-616

[6] IDENTIFICATION OF THE MITOCHONDRIAL RECEPTOR COMPLEX IN SACCHAROMYCES-CEREVISIAE [J].

MOCZKO, M ;

DIETMEIER, K ;

SOLLNER, T ;

SEGUI, B ;

STEGER, HF ;

NEUPERT, W ;

PFANNER, N .

FEBS LETTERS, 1992, 310 (03) :265-268

[7]

PFALLER R, 1989, J BIOL CHEM, V264, P34

[8] MITOCHONDRIAL PROTEIN IMPORT - NUCLEOSIDE TRIPHOSPHATES ARE INVOLVED IN CONFERRING IMPORT-COMPETENCE TO PRECURSORS [J].

PFANNER, N ;

TROPSCHUG, M ;

NEUPERT, W .

CELL, 1987, 49 (06) :815-823

[9] MITOCHONDRIAL IMPORT RECEPTORS FOR PRECURSOR PROTEINS [J].

PFANNER, N ;

SOLLNER, T ;

NEUPERT, W .

TRENDS IN BIOCHEMICAL SCIENCES, 1991, 16 (02) :63-67

[10] MITOCHONDRIAL PREPROTEINS EN-ROUTE FROM THE OUTER-MEMBRANE TO THE INNER MEMBRANE ARE EXPOSED TO THE INTERMEMBRANE SPACE [J].

RASSOW, J ;

PFANNER, N .

FEBS LETTERS, 1991, 293 (1-2) :85-88

← 1 2 →