CHEMICAL-STRUCTURE OF STEROLS THAT ACTIVATE OOCYTE MEIOSIS

被引：289

作者：

BYSKOV, AG ^{[1
]}

ANDERSEN, CY ^{[1
]}

NORDHOLM, L ^{[1
]}

THOGERSEN, H ^{[1
]}

XIA, GL ^{[1
]}

WASSMANN, O ^{[1
]}

ANDERSEN, JV ^{[1
]}

GUDDAL, E ^{[1
]}

ROED, T ^{[1
]}

机构：

[1] NOVO NORDISK AS, DK-2760 MALOV, DENMARK

来源：

NATURE | 1995年 / 374卷 / 6522期

关键词：

D O I：

10.1038/374559a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

GONADOTROPHINS and various growth factors, but not sex steroids, can induce resumption of meiosis in vitro, but only in oocytes enclosed by cumulus-granulosa cells(1). Follicular purines prevent resumption of meiosis(2,3). This process can be overcome, in vitro, by a transient elevation of cyclic AMP resulting in the production of a diffusible meiosis-inducing substance secreted by the cumulus cells(4). A meiosis-inducing activity has been detected in gonads of different species, for example, in preovulatory follicular fluid of women(5) and in mouse testes(6). We report here the isolation and characterization of meiosis-activating sterols from human follicular fluid and bull testes and the synthesis of two closely related C-29 sterols. All these sterols induce a resumption of meiosis in cultured cumulus-enclosed and naked mouse oocytes indicating their nonspecificity across species and sex. This family of sterols is for the first time considered crucial to meiosis.

引用

页码：559 / 562

页数：4

共 24 条

[1] INVITRO INHIBITION OF RAT-LIVER CHOLEST-5-EN-3-BETA-OL (CHOLESTEROL) BIOSYNTHESIS BY NON-MERCURIAL SULFHYDRYL REAGENTS [J].