THE USE OF CAPILLARY ELECTROPHORESIS FOR THE DETERMINATION OF HEMOGLOBIN-VARIANTS

被引：31

作者：

CASTAGNOLA, M

MESSANA, I

CASSIANO, L

RABINO, R

ROSSETTI, DV

GIARDINA, B

机构：

[1] CATHOLIC UNIV,INST CHEM & CLIN CHEM,ROME,ITALY

[2] CNR,CTR CHEM RECEPTORS & BIOL ACT MOLECULES,ROME,ITALY

来源：

ELECTROPHORESIS | 1995年 / 16卷 / 08期

关键词：

CAPILLARY ELECTROPHORESIS; CAPILLARY ZONE ELECTROPHORESIS; CAPILLARY ISOELECTRIC FOCUSING; MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY; HEMOGLOBIN VARIANTS;

D O I：

10.1002/elps.11501601247

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of capillary electrophoresis and related techniques for the detection of hemoglobin variants is described. Capillary zone electrophoresis (CZE) was applied for the analysis of intact tetrameric hemoglobin. CZE under denaturing conditions was used for the separation of globin chains. Both CZE and micellar electrokinetic capillary chromatography were applied for a fast and sensitive separation of tryptic digests and for the analysis of amino acid derivatives.

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页码：1492 / 1498

页数：7

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