HIGH-RESOLUTION 2-DIMENSIONAL GEL-ELECTROPHORESIS OF THE PROTEINS AND GLYCOPROTEINS OF HUMAN-BLOOD PLATELETS AND PLATELET MEMBRANES

被引：99

作者：

CLEMETSON, KJ ^{[1
]}

CAPITANIO, A ^{[1
]}

LUSCHER, EF ^{[1
]}

机构：

[1] HEMOPHILIA & THROMBOSIS CTR,I-20122 MILAN,ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 553卷 / 01期

关键词：

(Platelet); Membrane glycoprotein; Membrane protein; Two-dimensional gel electrophoresis;

D O I：

10.1016/0005-2736(79)90027-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The proteins and glycoproteins of human blood platelets and platelet membranes in both the reduced and the unreduced states have been analysed by isoelectric focusing and sodium dodecyl sulphate-discontinuous polyacrylamide gel electrophoresis in a two-dimensional technique. Gels which had been stained with periodic acid-Schiff's reagent could be counter-stained with Coomassie Brilliant Blue, simplifying the recognition of components which stain with both reagents. The major glycoproteins and some of the proteins have been identified and the characteristics of the membrane and of the whole platelet components established in this system. © 1979.

引用

页码：11 / 24

页数：14

共 26 条

[1] 2-DIMENSIONAL GEL-ELECTROPHORESIS OF MEMBRANE PROTEINS [J].