POTENTIOMETRIC ENZYME ELECTRODE FOR LACTATE

被引：35

作者：

SHINBO, T ^{[1
]}

SUGIURA, M ^{[1
]}

KAMO, N ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

ANALYTICAL CHEMISTRY | 1979年 / 51卷 / 01期

关键词：

D O I：

10.1021/ac50037a032

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A potentiometric enzyme electrode for lactate is developed. The enzyme electrode is constructed by coating the sensor membrane of the redox electrode with a film of enzyme-gelatin gel layer. The enzyme used is lactate dehydrogenase which catalyzes the oxidation of lactate by ferricyanide. The change in the concentration ratio of ferricyanide to ferrocyanide is monitored by the redox electrode which is a membrane electrode sensitive to redox potential of the solution. A plot of the potential of enzyme electrode vs. log [lactate] gives an S-shaped curve which depends on enzyme quantity and ferricyanide concentration. A relation between the enzyme electrode poteniial and substrate concentration is derived semitheoretically. The equation explains the results successfully. © 1979, American Chemical Society. All rights reserved.

引用

页码：100 / 104

页数：5

共 17 条

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