FURTHER-STUDIES ON THE TOPOGRAPHY OF THE N-TERMINAL REGION OF HUMAN PLATELET GLYCOPROTEIN-IIIA - LOCALIZATION OF MONOCLONAL-ANTIBODY EPITOPES AND THE PUTATIVE FIBRINOGEN-BINDING SITES

The precise localization of the epitopes for six monoclonal antibodies specific for the N-terminal region of human platelet glycoprotein IIIa (GPIIIa) was determined. The epitope for P37, a monoclonal antibody that inhibits platelet aggregation, was found at GPIIIa 101-109, flanked by the epitopes for P23-3 (GPIIIa 16-28), P23-4 (GPIIIa 83-91), P23-5 (GPIIIa 67-73), P23-7 (GPIIIa 114-122) and P40 (GPIIIa 262-302), and very close to the early chymotryptic cleavage site of GPIIIa in whole platelets (Phe-100). When the amino acid sequence of GPIIIa was searched for peptide sequence hydropathically complementary to the fibrinogen gamma-chain C-terminal (gamma 400-411) and A-alpha-chain RGD-containing peptides, none was found for the gamma-400-411, two (GPIIIa 128-132 and 380-384) were found complementary to fibrinogen A-alpha-574-575 and two (GPIIIa 109-113 and 129-133) were found for A-alpha-94-99. Two of these putative fibrinogen-binding sites overlap with each other, and a third one overlaps with the epitope for P37. These findings reinforce the earlier suggestion that the N-terminal region of GPIIIa is involved in fibrinogen binding, and suggest the existence in GPIIIa of either multiple or alternative RGD-binding sites or one RGD-binding domain with several moieties. Finally, early chymotryptic cleavage of GPIIIa in whole platelets liberates to the soluble fraction the peptide stretch Ser-101-Tyr-348, which carries the epitope for P37 and the putative binding sites for fibrinogen. The rest of the molecule, together with the GPIIb-resistant moiety, remains membrane-bound. This leads us to propose that the fibrinogen-binding domain of GPIIIa is not involved in the binding to GPIIb to form the Ca2+-dependent GPIIb-GPIIIa complex.