RAPID CHEMICAL-SAMPLING OF ENDOGENOUS SPECIES FROM BRAIN SLICE PREPARATIONS

被引：5

作者：

SHIEKHATTAR, R

BIBBS, EG

SCHOWEN, RL

ADAMS, RN

机构：

[1] UNIV KANSAS,DEPT CHEM,LAWRENCE,KS 66045

[2] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66045

[3] UNIV KANSAS,DEPT BIOCHEM,LAWRENCE,KS 66045

来源：

NEUROSCIENCE LETTERS | 1990年 / 111卷 / 1-2期

关键词：

Brain slice; Neurotransmitter release; Rapid chemical sampling;

D O I：

10.1016/0304-3940(90)90366-H

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

A very simple method of chemical sampling from the surface of brain slices is described. This procedure utilizing micropipets involves no sample dilution, and thus has very high sensitivity. Basal and stimulated concentrations of neurotransmitters and their metabolites are sampled from a thin fluid layer on the slice surface and appear to be in mobile equilibrium with the brain slice extracellular fluid levels. Rapid sampling allows one to follow the time course of stimulated release and reuptake phenomena. © 1990.

引用

页码：189 / 194

页数：6

共 10 条

[1] RAPID ANALYSIS OF AMINO-ACIDS USING PRE-COLUMN DERIVATIZATION [J].