EFFECT OF LIPASE SPECIFICITY ON TRIGLYCERIDE SYNTHESIS

被引:20
作者
ERGAN, F
TRANI, M
机构
[1] National Research Council Canada, Biotechnology Research Institute, Montréal
关键词
D O I
10.1007/BF01033510
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Enzymatic triglyceride synthesis from free fatty acid and glycerol is shown to be catalysed by both 1,3-specific lipase and non-specific lipase. This paper elucidates the mechanism of the reaction, showing that, with a 1,3-specific lipase, the 1,3-diolein enzymatically formed has to isomerise spontaneously to 1,2-diolein in presence of oleic acid, before being transformed to triolein by the enzyme.
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页码:19 / 24
页数:6
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