MAMMALIAN DNA- MUTANT DECREASING NUCLEAR-DNA POLYMERASE-ALPHA ACTIVITY AT NON-PERMISSIVE TEMPERATURE

被引：26

作者：

TSAI, YJ

HANAOKA, F

NAKANO, MM

YAMADA, M

机构：

[1] Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences University of Tokyo, Hongo, Tokyo

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期

关键词：

D O I：

10.1016/0006-291X(79)92005-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A temperature-sensitive Dna- mutant (ts T244) of the mouse FM3A cell line was characterized. ts T244 showed no increase in cell number and rapid decrease in the rate of DNA synthesis after temperature upshift (33°C→39°C). The activity of nuclear DNA polymerase α decreased as rapidly as the rate of DNA synthesis, whereas the activities of thymidine kinase and thymidylate kinase decreased more slowly. The results suggest that the level of nuclear DNA polymerase α has close relation to the ts defect of this Dna- mutant. © 1979.

引用

页码：1190 / 1195

页数：6

共 13 条

[1]

APOSHIAN HV, 1962, J BIOL CHEM, V237, P519

[2]

BRESNICK E, 1964, CANCER RES, V24, P841

[3] MAMMALIAN-CELL MUTANT WITH TEMPERATURE-SENSITIVE THYMIDINE KINASE [J].