MITOCHONDRIAL OXIDATIVE-PHOSPHORYLATION IN SAPONIN-SKINNED HUMAN MUSCLE-FIBERS IN STIMULATED BY CAFFEINE

被引：24

作者：

KUNZ, WS ^{[1
]}

KUZNETSOV, AV ^{[1
]}

GELLERICH, FN ^{[1
]}

机构：

[1] MOSCOW CARIOL RES CTR,BIOENERGET LAB,MOSCOW,RUSSIA

来源：

FEBS LETTERS | 1993年 / 323卷 / 1-2期

关键词：

SAPONIN-SKINNED MUSCLE FIBER; HUMAN SKELETAL MUSCLE; OXIDATIVE PHOSPHORYLATION; CAFFEINE;

D O I：

10.1016/0014-5793(93)81477-H

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The addition of 15 mM caffeine to saponin-skinned human muscle fibers from M. vastus lateralis caused in the presence of 2 mM ATP an approx. 2-fold stimulation of respiration with glutamate + malate. This effect can be abolished by either the addition of the Ca2+ chelator EGTA, the inhibitor of Ca2+ transport Ruthenium red and the inhibitor of the myosin ATPase vanadate. The caffeine concentration dependency of respiration of fibers coincided with the caffeine-caused stimulation of myosin ATPase activity. The activation of oxidative phosphorylation in saponin-skinned human muscle fibers by caffeine can be explained by a stimulation of myosin ATPase caused by Ca2+ release from sarcoplasmic reticulum.

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页码：188 / 190

页数：3

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