MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE PECTATE LYASE GENE FROM PSEUDOMONAS-MARGINALIS N6301

被引：15

作者：

NIKAIDOU, N ^{[1
]}

KAMIO, Y ^{[1
]}

IZAKI, K ^{[1
]}

机构：

[1] TOHOKU UNIV, FAC AGR, DEPT APPL BIOL CHEM, SENDAI 981, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 06期

关键词：

D O I：

10.1271/bbb.57.957

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pectate lyase was purified approximately 29-fold to electrophoretic homogeneity from Pseudomonas marginalis N6301. A pectate lyase (PL; EC4.2.2.2) gene of the strain was cloned and expressed in Escherichia coli. The nucleotides of the PL gene (pel) were sequenced. An open reading frame that encodes a polypeptide (molecular weight: 40,812) composed of 380 amino acids including a 29 amino acid signal peptide was assigned. The structural gene of pel consisted of 1140 base pairs. The nucleotide sequence of the 5'-flanking region of pel showed a consensus sequence of the promoter region of the pectin lyase gene (pnl) in P. marginalis N6301, a Pribnow box, and a ribosome binding site as found in E. coli.

引用

页码：957 / 960

页数：4

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