SEQUENCE ORGANIZATION OF THE HUMAN-CHROMOSOME 2Q TELOMERE

被引：53

作者：

MACINA, RA

NEGOREV, DG

SPAIS, C

RUTHIG, LA

HU, XL

RIETHMAN, HC

机构：

[1] WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104

[2] RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA

来源：

HUMAN MOLECULAR GENETICS | 1994年 / 3卷 / 10期

关键词：

D O I：

10.1093/hmg/3.10.1847

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The terminal 240 kb of a human 2q telomere region was cloned in two overlapping yeast artificial-chromosomes (YACs). This DNA contains a region of low-copy subtelomeric repeats (within 50 kb of the 2q telomere), a segment of DNA duplicated on distal 8p23 (100 kb from the 2q telomere), and a region of single-copy DNA (230 kb from the 2q telomere). Two CpG islands are present in the DNA segment duplicated on distal 8p23. RecA-assisted restriction endonuclease cleavage of genomic DNA samples revealed a potential 55 kb chromosome length polymorphism at the 2q telomere. This work provides telomeric closure of maps for human chromosome 2q, demonstrates a novel, subtelomere-specific DNA duplication, and will permit detailed molecular and cytological studies of this human telomere region.

引用

页码：1847 / 1853

页数：7

共 36 条

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