MODIFICATION OF ISLET OF LANGERHANS SURFACES WITH IMMUNOPROTECTIVE POLY(ETHYLENE GLYCOL) COATINGS VIA INTERFACIAL PHOTOPOLYMERIZATION

Poly(ethylene glycol) (PEG) has been used previously to alter immune interactions and systemic clearance of therapeutic proteins. We present herein chemical approaches for the conceptually similar treatment of therapeutic cells and tissues whereby immune and cell adhesive interactions may be reduced or interrupted, in the context of the transplantation of xenogeneic islets of Langerhans for the treatment of insulin-dependent diabetes mellitus. Visible-light-initiated interfacial photopolymerization of multifunctional PEG-based macromers was performed directly upon the surface of rat islets of Langerhans to produce conformal barrier hydrogel coatings with thickness of order 10 mu m. The islets continued to be normal in ultrastructure and function as reflected by response to a glucose challenge in vitro. (C) 1994 John Wiley & Sons, Inc.