SEPARATION AND QUANTITATION OF ESTERIFIED ESTROGENS IN BULK MIXTURES AND COMBINATION-DRUG PREPARATIONS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:16
作者
CAPITANO, G
TSCHERNE, R
机构
[1] Quality Control Department, Huffmann-La Roche, Inc., Nutley, New Jersey
关键词
Estrogens; esterified—high‐performance liquid chromatographic analysis in prepared solutions; High‐performance liquid chromatography—analysis; esterified estrogens in prepared solutions; Hormones—esterified estrogens; high‐performance liquid chromatographic analysis in prepared solutions;
D O I
10.1002/jps.2600680314
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
A high‐performance liquid chromatographic method for sterified estrogens is described. By using a facile acid hydrolysis extraction procedure for the sample preparation, the compounds are chromatographed as their free phenolic forms. The separation of tructurally similar compounds, such as equilenin, equilin, estione, and stradiol, was achieved with a reversed‐phase column and a methanol‐water mobile phase. Several samples of bulk mixtures and tablets were ssayed; the results compared favorably with those obtained using the JSP XIX method. The method was rapid, and the detector response was inear over a wide concentration range. A relative standard deviation of ±5% indicates the reliability and accuracy of the proposed method. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company
引用
收藏
页码:311 / 314
页数:4
相关论文
共 25 条
[1]   HIGH-SPEED LIQUID CHROMATOGRAPHIC SEPARATION OF EQUINE ESTROGENS [J].
BUTTERFIELD, AG ;
LODGE, BA ;
POUND, NJ .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1973, 11 (08) :401-405
[2]   ANALYSIS OF CONJUGATED ESTROGEN PREPARATIONS [J].
CAROL, J ;
BANES, D ;
GRAHAM, JH ;
KUNZE, FM .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1961, 50 (07) :550-&
[3]  
DISHMAN RA, 1970, THESIS U MASSACHUSET
[4]   ANALYSIS OF ESTROGENIC STEROIDS IN URINE BY HIGH-SPEED LIQUID CHROMATOGRAPHY [J].
DOLPHIN, RJ .
JOURNAL OF CHROMATOGRAPHY, 1973, 83 (AUG29) :421-429
[5]  
FIESER LF, 1959, STEROIDS, P477
[6]   ANALYSIS OF STEROIDS AND DERIVATIZED STEROIDS BY HIGH SPEED LIFQUID CHROMATOGRAPHY [J].
HENRY, RA ;
SCHMIT, JA ;
DIECKMAN, JF .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1971, 9 (09) :513-&
[7]  
HOUGH L, 1962, METHODS CARBOHYDRATE, V1, P21
[8]   SEPARATION OF SOME ESTROGEN SULFATES FROM THEIR OXIDATION PRODUCTS [J].
HURWITZ, AR ;
BURKE, HJ ;
MARRA, RA .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1967, 56 (11) :1509-&
[9]   SEPARATION OF CARBOHYDRATES AND PHOSPHORIC ESTERS ON PAPER CHROMATOGRAMS [J].
ISHERWOOD, FA .
BRITISH MEDICAL BULLETIN, 1954, 10 (03) :202-210
[10]   QUANTITATIVE GLC DETERMINATION OF CONJUGATED ESTROGENS IN RAW-MATERIALS AND FINISHED DOSAGE FORMS [J].
JOHNSON, R ;
MASSERANO, R ;
HARING, R ;
KHO, B ;
SCHILLING, G .
JOURNAL OF PHARMACEUTICAL SCIENCES, 1975, 64 (06) :1007-1011