SEPARATION AND QUANTITATION OF ESTERIFIED ESTROGENS IN BULK MIXTURES AND COMBINATION-DRUG PREPARATIONS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A high‐performance liquid chromatographic method for sterified estrogens is described. By using a facile acid hydrolysis extraction procedure for the sample preparation, the compounds are chromatographed as their free phenolic forms. The separation of tructurally similar compounds, such as equilenin, equilin, estione, and stradiol, was achieved with a reversed‐phase column and a methanol‐water mobile phase. Several samples of bulk mixtures and tablets were ssayed; the results compared favorably with those obtained using the JSP XIX method. The method was rapid, and the detector response was inear over a wide concentration range. A relative standard deviation of ±5% indicates the reliability and accuracy of the proposed method. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company