VOLTAMMETRIC AND SPECTROSCOPIC STUDIES OF PYRROLOQUINOLINE QUINONE COENZYME UNDER NEUTRAL AND BASIC CONDITIONS

被引：44

作者：

KANO, K ^{[1
]}

MORI, K ^{[1
]}

UNO, B ^{[1
]}

KUBOTA, T ^{[1
]}

IKEDA, T ^{[1
]}

SENDA, M ^{[1
]}

机构：

[1] KYOTO UNIV, FAC AGR, DEPT AGR CHEM, KYOTO 606, JAPAN

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1990年 / 23卷 / 03期

关键词：

D O I：

10.1016/0302-4598(90)80012-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The voltammetric and spectroscopic properties of pyrroloquinoline quinone (PQQ) have been investigated in the pH range from 5 to 13.5. PQQ gives a reversible cyclic voltammogram at the mercury electrode, which is ascribed to a two-step one-electron redox reaction of the o-quinone moiety. Digital simulation analysis of the reversible voltammogram made it possible to evaluate the standard redox potentials of the PQQ/pyrroloquinoline semiquinone (PQQsem) and PQQsem/pyyroloquinoline quinol (PQQred) couples. From the redox potential vs. pH relationships, the acid dissociation constants of PQQ, PQQsem, and PQQred have also been determined. The absorption and electron spin resonance spectroscopic properties are explained by the acid-base and redox equilibria proposed here. © 1990.

引用

页码：227 / 238

页数：12

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