CONCENTRATION OF TOCOPHEROLS FROM SOYBEAN SLUDGE BY SUPERCRITICAL CARBON-DIOXIDE

A supercritical fluid extraction method has been applied to test the feasibility of tocopherol concentration from soybean sludge with carbon dioxide at temperatures and pressures ranging from 35 to 70-degrees-C and 200 to 400 bar, respectively. The supercritical solubility of the esterified soybean sludge was over 4-6 times greater than that of the original soybean sludge. By a simple batch-type one-stage method the tocopherols in the esterified soybean sludge could be concentrated up to 40 wt%. The overall results of the present study show that soybean sludge initially containing about 13-14 wt% tocopherols may require a countercurrent multistage column to be highly and effectively concentrated.