DETERMINATION OF L-GLUTAMATE BY AMPEROMETRIC FLOW-INJECTION ANALYSIS USING IMMOBILIZED GLUTAMATE OXIDASE - MANIFOLD FOR SIMULTANEOUS DETECTION OF COMPONENT SIGNAL AND BLANK SIGNAL

被引：20

作者：

MATSUMOTO, K

SAKODA, K

OSAJIMA, Y

机构：

[1] Department of Food Science and Technology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka, 812, Hakozaki

来源：

ANALYTICA CHIMICA ACTA | 1992年 / 261卷 / 1-2期

关键词：

AMPEROMETRY; FLOW SYSTEM; FOODS; GLUTAMATE;

D O I：

10.1016/0003-2670(92)80186-B

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

L-Glutamate oxidase was immobilized on Amino-Cellulofine and used as an enzyme reactor in a flow-injection system. The hydrogen peroxide produced was monitored amperometrically. A new configuration is described for the determination of L-glutamate in food samples for which the matrix Provides varying blank values. The peak current was linearly related to L-glutamate concentration in the range 0.01-0.3 mM, and the relative standard deviation was 1.7% in ten successive assays at the 0.3 mM level. The proposed method was used for seasoning analysis and was compared with an L-glutamate kit (enzymatic, spectrophotometric) method.

引用

页码：155 / 159

页数：5

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