IDENTIFICATION OF A BENZHYDROLIC METABOLITE OF KETOPROFEN IN HORSES BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：13

作者：

BENOIT, E ^{[1
]}

JAUSSAUD, P ^{[1
]}

BESSE, S ^{[1
]}

VIDEMANN, B ^{[1
]}

COURTOT, D ^{[1
]}

DELATOUR, P ^{[1
]}

BONNAIRE, Y ^{[1
]}

机构：

[1] LAB,F-92290 CHATENAY MALABRY,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 583卷 / 02期

关键词：

D O I：

10.1016/0378-4347(92)80549-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A benzhydrolic metabolite of ketoprofen, formed by reduction of the keto group of the drug, has been identified by gas chromatography-mass spectrometry in equine plasma and urine. After partial synthesis, its structure has been confirmed by UV, IR and H-1 NMR spectroscopy. The kinetics of ketoprofen and this metabolite have been monitored in plasma by high-performance liquid chromatography. The two products were quantified in plasma up to 4 and 3 h, respectively, and were detected in urine up to 72 and 24 h, respectively, after a single intravenous administration to horses at the dose of 2.2 mg/kg. Simultaneous detection of both compounds increases the reliability of antidoping control analysis.

引用

页码：167 / 173

页数：7

共 26 条

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