EPITOPE MAPPING OF HUMAN ETS1 MONOCLONAL-ANTIBODY

被引：5

作者：

BHAT, NK ^{[1
]}

ROMANOSPICA, V ^{[1
]}

GEORGIOU, P ^{[1
]}

CHEN, SL ^{[1
]}

KUI, PG ^{[1
]}

SUZUKI, H ^{[1
]}

机构：

[1] PROGRAM RESOURCES INC,DYNCORP,FREDERICK,MD 21702

来源：

HYBRIDOMA | 1994年 / 13卷 / 01期

关键词：

D O I：

10.1089/hyb.1994.13.1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The epitope for E44 monoclonal antibody (mAb) was mapped using mutated ETS1 proteins lacking different carboxy-terminal regions and by the employment of synthetic oligopeptides spanning the epitope region. This epitope lies around Arg211 of the human ETS1 protein since substitution of Arg211 by Gln211 in the epitope region results in the loss of recognition of the mouse ETS1 protein by E44 mAb. Substitution of Leu214 by valine214 in the epitope region (as is found in the chicken ETS1 and viral Ets proteins) does not alter the capacity of the E44 mAb to recognize this antigen. Taken together, these results suggest that a specific ionic interaction is able to play a pivotal role in the recognition of the ETS1 protein by the E44 mAb.

引用

收藏

页码：1 / 8

页数：8

相关论文

共 50 条

[1] EXPRESSION OF ETS PROTO-ONCOGENES IN ASTROCYTES IN HUMAN CORTEX [J].

AMOUYEL, P ;

GEGONNE, A ;

DELACOURTE, A ;

DEFOSSEZ, A ;

STEHELIN, D .

BRAIN RESEARCH, 1988, 447 (01) :149-153

[2]

Ascione R., 1993, Genome research in molecular medicine and virology., P199

[3]

ASCIONE R, 1992, INT J ONCOL, V1, P631

[4]

Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210

[5] RECIPROCAL EXPRESSION OF HUMAN ETS1 AND ETS2 GENES DURING T-CELL ACTIVATION - REGULATORY ROLE FOR THE PROTOONCOGENE ETS1 [J].

BHAT, NK ;

THOMPSON, CB ;

LINDSTEN, T ;

JUNE, CH ;

FUJIWARA, S ;

KOIZUMI, S ;

FISHER, RJ ;

PAPAS, TS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (10) :3723-3727

[6]

BHAT NK, 1989, J IMMUNOL, V142, P672

[7] TEMPORAL AND TISSUE-SPECIFIC EXPRESSION OF MOUSE ETS GENES [J].

BHAT, NK ;

FISHER, RJ ;

FUJIWARA, S ;

ASCIONE, R ;

PAPAS, TS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (10) :3161-3165

[8] CHARACTERIZATION AND USES OF MONOCLONAL-ANTIBODY DERIVED AGAINST DNA-BINDING DOMAIN OF THE ETS FAMILY OF GENES [J].

BHAT, NK ;

PAPAS, TS .

HYBRIDOMA, 1992, 11 (03) :277-294

[9] SMALL REARRANGEMENTS IN STRUCTURES OF FV AND FAB FRAGMENTS OF ANTIBODY D1.3 ON ANTIGEN-BINDING [J].

BHAT, TN ;

BENTLEY, GA ;

FISCHMANN, TO ;

BOULOT, G ;

POLJAK, RJ .

NATURE, 1990, 347 (6292) :483-485

[10] THE PRODUCT OF THE C-ETS-1 PROTOONCOGENE AND THE RELATED ETS2 PROTEIN ACT AS TRANSCRIPTIONAL ACTIVATORS OF THE LONG TERMINAL REPEAT OF HUMAN T-CELL LEUKEMIA-VIRUS HTLV-1 [J].

BOSSELUT, R ;

DUVALL, JF ;

GEGONNE, A ;

BAILLY, M ;

HEMAR, A ;

BRADY, J ;

GHYSDAEL, J .

EMBO JOURNAL, 1990, 9 (10) :3137-3144

← 1 2 3 4 5 →