TYROSINASE-BASED RUTHENIUM DISPERSED CARBON-PASTE BIOSENSOR FOR PHENOLS

被引：78

作者：

WANG, J

LU, F

LOPEZ, D

机构：

[1] Department of Chemistry and Biochemistry, New Mexico State University, Las Cruces

来源：

BIOSENSORS & BIOELECTRONICS | 1994年 / 9卷 / 01期

关键词：

BIOSENSOR; PHENOL; TYROSINASE; ELECTROCATALYSIS; METALLIZED SURFACES;

D O I：

10.1016/0956-5663(94)80009-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Highly sensitive biosensors for phenolic compounds, based on the incorporation of the enzyme tyrosinase within metal-dispersed carbon paste matrices are described. In particular, ruthenium-doped carbon pastes display excellent electrocatalytic activity for the reduction of the enzymatically generated quinone species. Hence, convenient quantitation of micromolar concentrations is feasible at 0.0 V (vs. Ag/AgCl reference), with a greatly enhanced response compared to conventional carbon-paste tyrosinase biosensors. The influence of various experimental variables is explored. Fast, sensitive and reproducible flow injection detection of phenolic compounds is illustrated. Analogous improvements are reported for tyrosinase-rich plant tissue electrodes.

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页码：9 / 15

页数：7

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