IN-VIVO AND IN-VITRO EFFECTS OF WHEAT-GERM-AGGLUTININ AND BOWMAN-BIRK SOYBEAN TRYPSIN-INHIBITOR, 2 POTENTIAL TRANSGENE PRODUCTS, ON MIDGUT ESTERASE AND PROTEASE ACTIVITIES FROM APIS-MELLIFERA

Wheat germ agglutinin (WGA) and Bowman-Birk soybean trypsin inhibitor represent potential transgene products for inducing pest resistance in plants. The effects of these molecules were studied on midgut esterase and protease activities from Apis mellifera L., a major insect pollinator. Trypsin inhibitor and WGA did not exhibit an acute toxicity in A. mellifera. In vivo, trypsin inhibitor caused a decrease in the amount of trypsin activity and did not have a significant effect on esterase activity. In vitro, trypsin inhibitor inhibited about 80% of non-specific protease activity and 100% of trypsin activity. In vivo, WGA at high concentration in food (1 mg/ml) elicited a large decrease in trypsin activity and did not have a significant effect on esterase activity. In vitro, WGA did not have an significant effect on trypsin and non-specific protease activities but slightly activated esterase activity.