RADIOIMMUNOASSAY OF CORTICOSTERONE, CORTISOL AND CORTISONE - THEIR APPLICATION TO HUMAN CORD AND MATERNAL PLASMA

被引：67

作者：

SULON, J

DEMEYPONSART, L

BEAUDUIN, P

SODOYEZ, JC

机构：

[1] Département de Clinique, Pathologie Médicales (Prof. H. Van Cauwenberge) Université de Liège 1

来源：

JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | 1978年 / 9卷 / 07期

关键词：

D O I：

10.1016/0022-4731(78)90180-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A critical analysis of the applicability of corticosterone, cortisone and cortisol radioimmunoassays in human plasma is presented. Rabbits immunized with corticosteroid-3-CMO-BSA conjugates yielded specific antisera. Yet, their specificity may become insufficient when the concentrations of weakly cross-reacting steroids are much higher than that of the steroid to be assayed. In these particular instances, appropriate and simple purification steps, such as hexane extraction or celite chromatography, must be added. Normal values for pregnant women at delivery and their babies are presented. © 1978.

引用

页码：671 / 676

页数：6

共 35 条

[1]

ABRAHAM GE, 1975, PATHOL BIOL, V23, P889

[2]

Abraham GE, 1973, MODERN METHODS STERO, P451

[3] METABOLIC CLEARANCE RATE, BLOOD PRODUCTION, INTERCONVERSION AND TRANSPLACENTAL PASSAGE OF CORTISOL AND CORTISONE IN PREGNANCY NEAR TERM [J].