HIGH-FREQUENCY TRANSFORMATION OF BACILLUS-SUBTILIS PROTOPLASTS BY PLASMID DNA

被引：1005

作者：

CHANG, S ^{[1
]}

COHEN, SN ^{[1
]}

机构：

[1] STANFORD UNIV,SCH MED,DEPT MED,STANFORD,CA 94305

来源：

MOLECULAR & GENERAL GENETICS | 1979年 / 168卷 / 01期

关键词：

D O I：

10.1007/BF00267940

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycolinduced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4x107 transformants per μg of supercoiled DNA. Plasmids constructed by in vitro ligation or endonuclease-generated fragments of linear plasmid DNA can also transform PEG-treated protoplasts, but at a lower frequency. © 1979 Springer-Verlag.

引用

页码：111 / 115

页数：5

共 23 条

[1] GENERAL METHOD FOR INSERTING SPECIFIC DNA SEQUENCES INTO CLONING VEHICLES
BAHL, CP
MARIANS, KJ
WU, R
STAWINSKI, J
NARANG, SA
[J]. GENE, 1976, 1 (01) : 81 - 92
[2] BACTERIOCIN AND ANTIBIOTIC-RESISTANCE PLASMIDS IN BACILLUS-CEREUS AND BACILLUS-SUBTILIS
BERNHARD, K
SCHREMPF, H
GOEBEL, W
[J]. JOURNAL OF BACTERIOLOGY, 1978, 133 (02) : 897 - 903
[3] TRANSFORMATION OF BACILLUS-SUBTILIS - TRANSFORMING ABILITY OF DEOXYRIBONUCLEIC-ACID IN LYSATES OF L-FORMS OR PROTOPLASTS
BETTINGER, GE
YOUNG, FE
[J]. JOURNAL OF BACTERIOLOGY, 1975, 122 (03) : 987 - 993
[4] BIBB MJ, 1978, NATURE LONDON, V274, P388
[5] NONCHROMOSOMAL ANTIBIOTIC RESISTANCE IN BACTERIA - GENETIC TRANSFORMATION OF ESCHERICHIA-COLI BY R-FACTOR DNA
COHEN, SN
CHANG, ACY
HSU, L
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (08) : 2110 - &
[6] CONSTRUCTION OF BIOLOGICALLY FUNCTIONAL BACTERIAL PLASMIDS IN-VITRO
COHEN, SN
CHANG, ACY
BOYER, HW
HELLING, RB
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (11) : 3240 - 3244
[7] DUBNAU D, 1976, MICROBIOLOGY 1976, P14
[8] MECHANISM OF INTEGRATING FOREIGN DNA DURING TRANSFORMATION OF BACILLUS-SUBTILIS
DUNCAN, CH
WILSON, GA
YOUNG, FE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (08) : 3664 - 3668
[9] EHRILICH SD, 1978, P NATL ACAD SCI US, V75, P1433
[10] REPLICATION AND EXPRESSION OF PLASMIDS FROM STAPHYLOCOCCUS-AUREUS IN BACILLUS-SUBTILIS
EHRLICH, SD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (04) : 1680 - 1682

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