IMMOBILIZATION OF GLUCOSE-ISOMERASE ON INDION-48-R

被引：14

作者：

GAIKWAD, SM ^{[1
]}

DESHPANDE, VV ^{[1
]}

机构：

[1] NATL CHEM LAB,DIV BIOCHEM SCI,POONA 411008,MAHARASHTRA,INDIA

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1992年 / 14卷 / 10期

关键词：

GLUCOSE ISOMERASE; GLUCOSE; IMMOBILIZATION; FRUCTOSE; HIGH FRUCTOSE SYRUP (HFS);

D O I：

10.1016/0141-0229(92)90104-V

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The glucose isomerase from Streptomyces sp. NCIM 2730 was produced using wheat bran or bagasse hydrolyzate as an inducer and immobilized on an inexpensive anion exchange resin Indion 48-R. The adsorption capacity and efficiency of the resin were found to be 25 U g-1 and 60%, respectively. The properties of soluble and immobilized glucose isomerase were compared. The stability of the enzyme al higher temperatures and acidic pH is increased due to immobilization. Concentrated glucose syrup (25%) was efficiently converted to fructose by the immobilized enzyme. After the seventh reuse, the conversion capacity of immobilized glucose isomerase was reduced to 61%, with a 35% loss in enzyme activity.

引用

页码：855 / 858

页数：4

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