RAPID REGULATION OF CORTICOTROPIN-RELEASING HORMONE GENE-TRANSCRIPTION INVIVO

被引:174
作者
HERMAN, JP [1 ]
SCHAFER, MKH [1 ]
THOMPSON, RC [1 ]
WATSON, SJ [1 ]
机构
[1] UNIV MICHIGAN, MENTAL HLTH RES INST, ANN ARBOR, MI 48109 USA
关键词
D O I
10.1210/me.6.7.1061
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Regulation of corticotropin-releasing hormone (CRH) gene expression in vivo was assessed via in situ hybridization histochemistry, using probes directed against an intronic sequence of the CRH gene. Initial characterization of the CRH intron (CRHin) probe revealed specific localization of signal to the nuclear compartment of neurons in the medial parvocellular paraventricular hypothalamus, which are known to produce CRH peptide and mRNA. Abundance of CRHin signal was low, commensurate with a low resting pool of CRH heteronuclear RNA (hnRNA), representing CRH primary transcript. Regulation of CRH hnRNA levels was assessed after acute glucocorticoid synthesis blockade by injection of metyrapone. Metyrapone inhibits the conversion of 11-deoxycorticosterone to corticosterone, thereby rapidly depleting glucocorticoids and serving as a discrete stimulus for hypothalamo-pituitary-adrenocortical activation. Plasma hormone measurements verified the efficacy of treatment, as metyrapone-treated rats showed extremely low basal corticosterone levels at all postinjection time points, while exhibiting progressive increases in plasma ACTH release over the 60-min postinjection period. CRH hnRNA levels were markedly increased 15-30 min after metyrapone injection, consistent with a rapid induction of CRH gene transcription in response to the stimulatory event. CRH mRNA, on the other hand, did not exceed control levels until 60 min post metyrapone, illustrative of a temporal lag between transcriptional changes and detectable changes in mRNA pools. Additional sections from metyrapone- and vehicle-treated rats were hybridized with probes complementary to mRNA encoding the immediate-early gene c-fos. c-fos was not present under unstimulated conditions yet was rapidly induced upon metyrapone treatment or vehicle injection (15 min). Induction of c-fos was more prolonged in metyrapone-treated rats, indicative of the glucocorticoid depletion elicited by drug treatment. These studies indicate that in CRH neurons, discrete steroid depletion can induce a rapid increase in transcription of genes involved in both cellular activation (c-fos) and in synthesis of secretory products (CRH).
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页码:1061 / 1069
页数:9
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