PROTEOLYTIC PROCESSING OF HUMAN INTESTINAL LACTASE-PHLORHIZIN HYDROLASE PRECURSOR IS NOT A PREREQUISITE FOR CORRECT SORTING IN MADIN DARBY CANINE KIDNEY (MDCK) CELLS

被引：18

作者：

GRUNBERG, J

LUGINBUHL, U

STERCHI, EE

机构：

[1] UNIV BERN, INST BIOCHEM & MOLEC BIOL, BUEHLSTR 28, CH-3012 BERN, SWITZERLAND

[2] UNIV BERN, FAC MED, DEPT PEDIAT, CH-3012 BERN, SWITZERLAND

来源：

FEBS LETTERS | 1992年 / 314卷 / 03期

关键词：

LACTASE-PHLORHIZIN HYDROLASE; HUMAN; ENTEROCYTE; PROTEOLYTIC PROCESSING; SORTING; SURFACE EXPRESSION;

D O I：

10.1016/0014-5793(92)81476-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Maturation of lactase-phlorizin hydrolase (LPH) (EC 3-2.1.23-62) requires proteolytic processing of precursor (pro-LPH) to mature microvillus membrane enzyme (m-LPH). Subcellular site and function of this processing are unknown. We studied the processing and sorting of human LPH expressed permanently in MDCK cellls. LPH was inserted into the apical membrane and small amounts were found basolateral. Of the LPH immunoprecipitated from the apical membrane, 42% was in the mature, i.e. proteoytically processed form; on the basolateral membrane it was 20%. Thus, LPH-processing occurs after sorting and is not necessary for surface expression.

引用

页码：224 / 228

页数：5

共 26 条

[1]

BULLER HA, 1987, J BIOL CHEM, V262, P17206

[2]

BULLER HA, 1989, AM J PHYSL, V257

[3]

CAPLAN M, 1989, FUNCTIONAL EPITHELIA

[4]

DANIELSEN EM, 1984, BIOCHEM BIOPH RES CO, V122, P82