GEOGRAPHICAL VARIATION IN 18S RIBOSOMAL-RNA GENE SEQUENCE OF CRYPTOSPORIDIUM-PARVUM

被引：18

作者：

KILANI, RT ^{[1
]}

WENMAN, WM ^{[1
]}

机构：

[1] WALTER MACKENZIE CTR,EDMONTON T6G 2J2,AB,CANADA

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1994年 / 24卷 / 02期

关键词：

CRYPTOSPORIDIUM PARVUM; APICOMPLEXA; 18S RIBOSOMAL RNA; DNA SEQUENCE; GEOGRAPHICAL VARIATION;

D O I：

10.1016/0020-7519(94)90044-2

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Using 18S rRNA as a probe, an EcoR1 fragment containing 1507 nt of 18S rRNA from C. parvum was identified, cloned and sequenced. Comparison of this sequence with the partial sequence of the small subunit rRNA of Cryptosporidium published by Johnson, Fielke, Lumb & Baverstock (International Journal for Parasitology 20: 141-147, 1990) and 1516 bp of the 18S rRNA nucleotide sequence of C. parvum published by Cai, Collins, McDonald and Thompson (Biochimica et Biophysica Acta, 1131: 317-320, 1992) revealed 97% and 91.6% sequence homology, respectively. These data suggest that differences exist among the same species of Cryptosporidium from different geographical areas.

引用

页码：303 / 306

页数：4

共 13 条

[1] PCR CLONING AND NUCLEOTIDE-SEQUENCE DETERMINATION OF THE 18S RIBOSOMAL-RNA GENES AND INTERNAL TRANSCRIBED SPACER-1 OF THE PROTOZOAN PARASITES CRYPTOSPORIDIUM-PARVUM AND CRYPTOSPORIDIUM-MURIS [J].