PREPARATION AND USE OF A MONOCLONAL-ANTIBODY TO DETECT CHLAMYDIA-PSITTACI ANTIGEN IN PARAFFIN-EMBEDDED TISSUE-SECTIONS

被引：3

作者：

BOLLO, E

BIOLATTI, B

DONN, A

TURILLI, C

WILSMORE, AJ

机构：

[1] ROYAL VET COLL,DEPT ANIM HLTH,POTTERS BAR EN6 1NB,HERTS,ENGLAND

[2] IST ZOOPROFILATT SPERIMENTALE VENEZIE,I-35100 PADUA,ITALY

来源：

RESEARCH IN VETERINARY SCIENCE | 1992年 / 53卷 / 03期

关键词：

D O I：

10.1016/0034-5288(92)90146-S

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A murine monoclonal antibody prepared against an ovine abortion isolate of Chlamydia psittaci (A22/Teramo) revealed specific binding to a 57 kDa chlamydial antigen in immunoblotting studies. The monoclonal antibody was able to detect intracytoplasmic chlamydial inclusions and scattered elementary bodies in infected McCoy cell culture, and on formalin-fixed paraffin-embedded tissue sections both from experimentally infected mice and from fetal membranes of cases of ovine enzootic abortion.

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页码：393 / 396

页数：4

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