GENETIC-EVIDENCE FOR THE INTERACTION OF THE YEAST TRANSCRIPTIONAL COACTIVATOR PROTEINS GCN5 AND ADA2

被引:57
作者
GEORGAKOPOULOS, T
GOUNALAKI, N
THIREOS, G
机构
[1] FDN RES & TECHNOL HELLAS, INST MOLEC BIOL & BIOTECHNOL, GR-71110 IRAKLION, GREECE
[2] UNIV CRETE, DEPT BIOL, GR-71110 IRAKLION, GREECE
来源
MOLECULAR AND GENERAL GENETICS | 1995年 / 246卷 / 06期
关键词
TRANSCRIPTIONAL COACTIVATORS; GCN5; ADA2; BROMODOMAIN;
D O I
10.1007/BF00290718
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The GCN5 and ADA2 proteins are required for the activation function of a number of transcriptional activators in the yeast Saccharomyces cerevisiae. By using appropriate LexA fusion proteins we demonstrated that both proteins are required for part of the function of the GCN4, GAL4 and the VP16 transcriptional activation domains. Analysis of a gcn5 ada2 double disruption mutant did not reveal any additive effects, suggesting that the two proteins act in the same pathway. The GCN5 and ADA2 proteins can each activate transcription when directed to the promoter region of a reporter gene, but only in the presence of a wild-type ADA2 or GCN5 gene, respectively. The activation capacity is enhanced when the corresponding endogenous gene copy is disrupted. Taken together, these genetic data suggest that the two proteins interact and define one complex that mediates transcriptional activation. The function of this complex requires the bromodomain region of the GCN5 protein.
引用
收藏
页码:723 / 728
页数:6
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