REVERSED IMMUNOSORBENTS - SIMPLE METHOD FOR SPECIFIC ANTIBODY IMMOBILIZATION

被引：15

作者：

LEVY, DE ^{[1
]}

EVELEIGH, JW ^{[1
]}

机构：

[1] OAK RIDGE NATL LAB,DIV BIOL,OAK RIDGE,TN 37830

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1978年 / 22卷 / 1-2期

关键词：

D O I：

10.1016/0022-1759(78)90065-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is presented for permanently converting an antigen immunosorbent into a purified antibody immunosorbent which retains specific reactivity for antigen. Following immunological reaction of the antibodies in an antiserum with the antigen adsorbent, the specific immunoglobulin is chemically bound to the antigen by means of a divalent cross-linking agent. Various such agents, including glutaraldehyde, suberimidate, a diazide, and isocyanates, were employed. The conditions of preparation using the first two were optimized, and the characteristics of the resulting immobilized antibodies were investigated. Applications in radioimmunoassay, preparative chromatography, and affinity-constant studies are discussed in view of the unique attributes of these reagents. © 1978.

引用

页码：131 / 142

页数：12

共 10 条

[1] ANALYTICAL TECHNIQUES FOR CELL FRACTIONS .20. CYCLIC AFFINITY CHROMATOGRAPHY - PRINCIPLES AND APPLICATIONS [J].