RAPID-DETERMINATION OF METHYLATED PURINES IN DNA TREATED WITH N-METHYL-N-NITROSOUREA USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：13

作者：

THIELMANN, HW

机构：

[1] Deutsches Krebsforschungszentrum, Institut für Biochemie

来源：

CANCER LETTERS | 1979年 / 6卷 / 06期

关键词：

D O I：

10.1016/S0304-3835(79)80087-7

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A method for determining methylated purine bases in [3H]N-methyl-N-nitrosourea (MeNOUr) treated DNA is described. The method combines reversed-phase high-performance liquid chromatography (HPLC) of methylated DNA after hydrolysis in dilute acid with the determination of radioactivity in the fractionated eluates. The peaks of the respective methylated purines were indentified by the use of internal standards. The method allows quantitative separation of 3-methyl-adenine (m3 Ade), 7-methyl-adenine (m7 Ade), 3-methyl-guanine (m3 Gua), 7-methyl-guanine (m7 Gua) and O6-methyl-guanine (m6 Gua) within 20 min. Thus the total time required for determination of methylated purines is limited only by radioactivity measurements in the respective fractions. © 1979 Elsevier/North-Holland Scientific Publishers Ltd., All rights reserved.

引用

页码：311 / 317

页数：7

共 12 条

[1]

BOCHERT G, 1977, METHODS PRENATAL TOX, P456

[2] SELECTIVE ACCUMULATION OF O-6-METHYLGUANINE IN DNA OF RAT BLADDER EPITHELIUM AFTER INTRAVESICAL ADMINISTRATION OF N-METHYL-N-NITROSOUREA [J].