MGATP-INDUCED INHIBITION OF THE ENZYMIC ACTIVITY OF CHLOROFORM-RELEASED OX-HEART MITOCHONDRIAL ATPASE

被引：12

作者：

LOWE, PN

LINNETT, PE

BAUM, H

BEECHEY, RB

机构：

[1] UNIV LONDON CHELSEA COLL SCI & TECHNOL,LONDON SW3 6LX,ENGLAND

[2] SHELL RES LTD,SITTINGBOURNE RES CTR,BIOSCI LAB,SITTINGBOURNE ME9 8AG,KENT,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)91564-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Preincubation of the chloroform-released ox-heart mitochondrial ATPase with its substrate, MgATP, results in a time-dependent inhibition of its ATPase activity. The inhibition is irreversible on the time-scale of an ATPase assay. It is not due to the accumulation of ADP and Pi. The extent of the inhibition is proportional to the number of turnovers of the enzyme during the preincubation period. It is suggested that the MgATP-induced inhibition described here is due to an intermediate enzyme-substrate complex of the ATP-hydrolytic pathway becoming converted into an inhibited enzyme species. © 1979.

引用

页码：599 / 605

页数：7

共 14 条

[1] ELECTROPHORETIC MICROHETEROGENEITY AND SUBUNIT COMPOSITION OF 13S COUPLING FACTORS OF OXIDATIVE AND PHOTOSYNTHETIC PHOSPHORYLATION [J].